Concentration

Data Sheet: Activity – Photosynthesis and Respiration

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Name

Course

Date

Activity Data Code

Procedure I – Net Photosynthesis Rates – 100% Illumination

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Leaf

Sample

Type

Illumination

Level

Oxygen

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Oxygen

Change Rate

(ppm/s)

Sun-

Adapted

100%

Shade-Adapted

100%

Leaf

Sample

Type

Illumination

Level

Carbon Dioxide

Concentration

Change Data

(ppm)

Elapsed

Time

(s)

Carbon Dioxide

Change Rate

(ppm/s)

Sun-

Adapted

100%

Shade-Adapted

100%

Observations and Questions

[1] How is chlorophyll involved during photosynthesis?

[2] What are enzymes? What role do they play in biological and chemical reactions?

[3] How might differences in enzyme concentrations affect rates of photosynthesis and respiration?

[4] Show your oxygen change rate calculation for the Sun-Adapted data run.

[5] Show your carbon dioxide change rate calculation for the Shade-Adapted data run.

[6] Review the chemical reaction for photosynthesis and then carefully examine your results from Procedure I. Do your results support the relationship between oxygen and carbon dioxide shown in the chemical reaction? Explain. Cite your results as part of your explanation.

[7] Using your results for change in oxygen concentration as a reference, discuss whether your results from Procedure I confirm research findings about differences in the amount of chlorophyll production for sun-adapted and shade-adapted leaves.

[8] What is the difference between photosynthesis rate and net photosynthesis rate? Which rate are you determining in this procedure? Explain.

[9] Explain why (or why not) you would expect respiration to occur when the illumination is 100%?

Procedure II – Respiration Rates – 0% Illumination Data Run

Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Leaf

Sample

Type

Illumination

Level

Oxygen

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Oxygen

Change Rate

(ppm/s)

Sun-

Adapted

0%

Shade-Adapted

0%

Leaf

Sample

Type

Illumination

Level

Carbon Dioxide

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Carbon Dioxide

Change Rate

(ppm/s)

Sun-

Adapted

0%

Shade-Adapted

0%

Observations and Questions

[10] Sample Calculation: Show your oxygen change rate calculation for the Sun-Adapted data run.

[11] Sample Calculation: Show your carbon dioxide change rate calculation for the Shade-Adapted data run.

[12] What would be the photosynthesis rate if you measured it at 0% illumination in Procedure II? Explain your answer. Cite your results from Procedure I to assist with your explanation.

[13] Cite your results for the change in carbon dioxide concentration in Procedure II as a reference and compare the rates of cellular respiration for sun-adapted and shade-adapted leaves. Explain the biological principles/bases underlying this comparison.

Procedure III – Low-Light Net Photosynthesis Rates – 15% Illumination

Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)

Leaf

Sample

Type

Illumination

Level

Oxygen

Concentration

Change Data (ppm)

Elapsed

Time

(s)

Oxygen

Change Rate

(ppm/s)

Sun-

Adapted

15%

Shade-Adapted

15%

Observations and Questions

[14] Sample Calculation: Show your oxygen change rate calculation for the Sun-Adapted data run.

[15] Based on the data you collected in Procedures I (100% illumination) and III (15% illumination), which system of leaves produces the most oxygen: Sun-adapted or shade-adapted? Explain how your results support your answer.

[16] Are your results from Procedure II and Procedure III consistent with research findings that there are differences in chlorophyll production and enzyme activity between shade-adapted leaves and sun-adapted leaves? Explain your reasoning. Cite your results from Procedure II and Procedure III to support your explanation.

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Files: Activity-Form-PhotoSyn.docx
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